sCD59, Human, ELISA kit

Name: sCD59, Human, ELISA kit
SKU: HK374
Availability: InStock

€825.00 – €1,359.00

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Description
Application

CD59, an essential glycoprotein on cell surfaces, regulates complement-mediated lysis by inhibiting the membrane attack complex (MAC). As a guardian of cellular integrity, sCD59 prevents inflammation and thrombosis by blocking MAC formation. Its ubiquitous presence across mammalian cells underscores its role in immune regulation and T cell activation. CD59 gene mutations lead to deficiency, causing hemolytic anemia and cerebral infarction, highlighting its clinical importance. With levels ranging from 22 to 119 ng/ml in healthy plasma, sCD59 emerges as a crucial biomarker for immune health, offering therapeutic potential for complement-related disorders.

Application

The sCD59 ELISA kit is to be used for the in vitro quantitative determination of human sCD59 in plasma.

Principle

The sCD59 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human sCD59. Biotinylated tracer antibody will bind to the captured human sCD59. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human sCD59 standards (log). The human sCD59 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve.

Specifications
Downloads

Product type

Assays

Amount

1 x 96 det., 2 x 96 det.

Standard range

Measurable concentration range of 0.39 to 25 ng/ml.

Detection level

Minimum concentration which can be measured is 0.39 ng/ml.

Working volume

100 µl/well

Species

Human

Cross reactivity

Mouse – No, Pig – No, Rat – No

Alias

CD59 glycoprotein, MAC-inhibitory protein, MAC-IP, membrane inhibitor of reactive lysis, MIRL, Protectin

Storage and stability

Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Disease

Infectious diseases, Neurological disorders

Assay Manual